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Description
Human Cath-V ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Cathepsin V (Cath-V) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the Cathepsin V (Cath-V) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Cathepsin V ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Cathepsin V (also known as cathepsin L2 or cathepsin U) is a protein encoded by the CTSV gene. This protein is a cysteine protease and a lysosomal cysteine protease with endopeptidase activity. It is a member of the papain family (MEROPS family C1) and a lysosomal cysteine protease with endopeptidase activity. It may play an important role in corneal physiology. This gene is expressed in colorectal and breast cancers but not in normal colon, breast, or peritumoral tissues, suggesting a possible role for this gene in neoplastic processes. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
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Product Reviews
★★★★★ 5
Nice sunscreen.
Great sunscreen. Works into skin without residue. I get good sun protection and lasts most of the day.
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Reviewed in the United States on April 3, 2026
★★★★★ 5
Best dry-touch broad spectrum sunscreen available.
I've use this for the past 10 years. It is highly effective. In addition, a small amount goes a long way and it is NOT GREASY!! That is a major plus for me. I keep a short beard and it is easy to use with the beard. It functions very well and I have not found a better product. Other than its effectiveness, you can't tell you used it.
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Reviewed in the United States on April 7, 2026
★★★★★ 5
It works
Best product to protect from the sun
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Reviewed in the United States on April 27, 2026
★★★★★ 5
Great sunscreen🌞
Neutrogena sunscreen is my favorite. It has never made me break out and use it on my face every day for years and never broke out from this brand. My doctor wanted me to use sunscreen 24/7 for years.
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Reviewed in the United States on May 8, 2026
★★★★★ 4
A Sunscreen That Checks Every Box!
I recently picked up the **Neutrogena Ultra Sheer Dry‑Touch Sunscreen Lotion **SPF 70 in the convenient travel‑size 3‑pack, and I couldn’t be more satisfied. Here’s why this product deserves every bit of its glowing reputation:
1. High End Sun Protection in Compact Form
With broad spectrum SPF 70, this sunscreen offers robust defense against both UVA (aging) and UVB (burning) rays, making it a reliable choice for daily city exposure or weekend adventures .The travel-sized 3 oz bottles are perfect for on the go use slip them into your gym bag, purse, or carry on without a hitch.
2. Dry Touch, Non Greasy Feel
The standout Dry Touch technology ensures a sheer, matte finish that absorbs almost instantly, leaving you feeling fresh rather than sticky or oily . Multiple reviewers on Amazon raved about its silky, non greasy texture, with one even saying “it goes on easy and did not feel greasy” .
3. Friendly for Sensitive & Acne Prone Skin
Designed to be noncomedogenic, PABA free, and oxybenzone free, this sunscreen avoids pore‑clogging and potential irritants . Many users noted no skin irritation or breakouts even those with combination or sensitive skin reported smooth, comfortable wear .
4. Water Resistant & Durable
Rated water resistant for up to 80 minutes, it holds its protective power well during sweating or light swimming, which is perfect for hot days, workouts, or errands in the sun .
5. Pleasant, Subtle Scent
This sunscreen carries a mild, fresh scent that’s noticeable but not overpowering. A few reviewers appreciated this clean fragrance profile, making application pleasant rather than perfumed .
6. Affordable & Practical Bundle
At roughly the price of a single regular-size bottle, the 3‑pack offers excellent value ideal for stocking up or sharing. Users frequently praise it as “a great value” and “perfect for a beach bag or purse” .
A Few Minor Things to Note
• Shake Before Use: Some buyers reported slight separation of formulas just give it a good shake to ensure consistent, smooth application.
• Occasional White Cast: A small number of users noted a subtle white cast, primarily if not well blended, but most say it blends out cleanly and is barely noticeable .
Final Verdict
Neutrogena Ultra Sheer Dry‑Touch SPF 70 (Travel‑Size 3 Pack) is a rare combo of high-level sun protection, everyday comfort, and great portability. It’s lightweight, dries almost like nothing is there, and won’t clog pores or leave a slick residue making it a go-to for work, travel, workouts, and weekends.
If you’re looking for a powerful yet elegant sunscreen that fits seamlessly into a busy lifestyle, this is absolutely it. I’m already repurchasing it and recommending it to everyone who asks!
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Reviewed in the United States on June 19, 2025
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