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Description
Human MMP-9 (Matrix Metalloproteinase 9) ELISA KitThe Human (MMP 9) Matrix Metalloproteinase 9 ELISA Kit measures Matrix Metalloproteinase 9 Gelatinase B in samples. The plate has been pre coated with Human MMP 9 antibody. MMP 9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MMP 9 Antibody is added and binds to MMP 9 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MMP 9 antibody. After incubation unbound Streptavidin
The Human (MMP-9) Matrix Metalloproteinase 9 ELISA Kit measures Matrix Metalloproteinase 9/Gelatinase B in samples. The plate has been pre-coated with Human MMP-9 antibody. MMP- 9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MMP-9 Antibody is added and binds to MMP-9 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MMP-9 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MMP-9. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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